Two ways to fold the genome during the cell cycle: insights obtained with chromosome conformation capture

two-ways

Genetic and epigenetic inheritance through mitosis is critical for dividing cells to maintain their state. This process occurs in the context of large-scale re-organization of chromosome conformation during prophase leading to the formation of mitotic chromosomes, and during the reformation of the interphase nucleus during telophase and early G1. This review highlights how recent studies over the last 5 years employing chromosome conformation capture combined with classical models of chromosome organization based on decades of microscopic observations, are providing new insights into the three-dimensional organization of chromatin inside the interphase nucleus and within mitotic chromosomes. One striking observation is that interphase … Read more!…

Nuclear Cartography: Techniques for mapping chromosome conformation

ENCODE

RESEARCHER: Job Dekker, Professor and Co-Director, Program in Systems Biology, University of Massachusetts Medical School, Worcester PROJECT: Mapping long-range DNA interactions as part of the ENCODE project PROBLEM: 3C is not easily multiplexed, making its application to large genomic surveys impractical. SOLUTION: 3C is sometimes called a one vs. one technique because it is used to measure individual interactions between selected pairs of genomic segments. In the technique, cellular chromatin structure is frozen in place with a crosslinker such as formaldehyde. Restriction enzymes cut the DNA and the resulting ends are then ligated together to physically link the two previously … Read more!…